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The supernatant obtained from the final enrichment step was filter sterilized through a 0.45 μm pore size syringe filter and checked for the presence of phages by the soft agar overlay method. The soft agar was prepared by adding 100 μl phage lysate to 200 μl of an overnight culture of indicator strain and mixed with 5 ml of liquid soft agar
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Mar 01, 2003 · PCR amplification was performed in 20-μl reaction volumes containing 14.1 μl dd H 2 O, 2 μl 10x buffer (Promega), 1.2 μl 25 mM MgCl 2, 0.5 μl dNTPs (10 mM each), 0.5 μl 20 pmol forward and reverse primer, 1 U of TaqDNA polymerase (Promega), and 1 μl of DNA template.PCR cycling was performed under conditions optimized for each primer pair as follows:35 cycles of denaturation at
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Based on these three parameters, the erythrocyte was estimated to be as much as 1000 times more susceptible to As(V) than As(III). ATP is required for the cell to maintain membrane integrity and
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Mar 05, 2019 · African populations have been underrepresented in human genomics research yet are important for understanding modern human origins and the genetic basis of adaptive traits. Here we analyze a genome-wide dataset in 840 ethnically and geographically diverse Africans. We find that geographically distant hunter-gatherer populations from East Africa share unique common ancestry
No cell phones allowed in the jail lobby. In Shawnee County there are a lot of bars, coffee shops, and parks. If you want to know if someone has been booked into the Shawnee County Jail system in Topeka, Kansas, you must call 785-291-5000 and press 3 when prompted. Please input inmate's name or booking number and state.
Between Nov 28, 2017, and Dec 4, 2017, of 2965 women assessed for eligibility, 2850 pregnant or recently pregnant women with suspected or confirmed infection were included. 70·4 (95% CI 67·7–73·1) hospitalised women per 1000 livebirths had a maternal infection, and 10·9 (9·8–12·0) women per 1000 livebirths presented with infection
WHEREAS Joan Sera Mjomba, is registered as proprietor in absolute ownership interest of all that piece of land containing 0.10 hectare or thereabouts, situate in the district of Taita Taveta, registered under title No. Taita Taveta/Voi/Bomani Phase 1/783, and whereas sufficient evidence has been adduced to show that the land title deed issued
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"Lexicon of a Classical Sub-Saharan Therapeutics" in chapter 2 was first put forward in a paper prepared for the Hamburg, Germany, conference on "Ethnomedicine and Medical History," May, 1980, organized by Joachim Sterly and Hans Morgenthaeler, and subsequently published as "Towards a Historical Perspective on African Medicine and Health" in Ethnomedizin und Medizingeschichte (1983).
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by Dia-Eldin A. Elnaiem, Osman Dakein, Ahmed Mohammed-Ali Alawad, Bashir Alsharif, Altayeb Khogali, Tayseer Jibreel, Omran F. Osman, Hassan Has ’san, Atia Mohamed Atia, Mousab Elhag, Margriet Den Boer, Koert Ritmeijer, Caryn Bern, Jorge Alvar, Noteila Khalid, Orin Courtenay Visceral Leishmaniasis (VL) due toLeishmania donovani is a neglected protozoan parasitic disease in humans, which is
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